Selective culture medium failure in gonorrhoea.

Diagnosis of gonorrhoea in women.

250 consecutive female contacts of men with gonorrhoea were examined in Lydia Department of Genito-urinary Medicine, St Thomas' Hospital, London, and 165 (66 per cent.) were found to be positive for gonorrhoea. Over 99 per cent. of the positive results were found by the first two sets of genital investigations. These results are compared with earlier and more recent results from our own and oth...

Diagnosis of gonorrhoea by culture on a selective medium containing vancomycin, colistin, nystatin and trimethoprim (VCNT). A comparison with Gram-staining and immunofluorescence.

Chacko-Nair egg-enriched selective medium in the diagnosis of pathogenic Neisseriae.

An egg-enriched medium made selective by the addition of Polymyxin B and Mycostatin, for the isolation of N. gonorrhoeae was described by Chacko and Nair (1966). The evaluation of this medium has since been extended to further cases ofN.gonorrhoeae and also to N. meningitidis. The results indicate the usefulness of the Chacko-Nair medium, not only in the diagnosis of gonorrhoea, but also in the...

Assessment of a selective medium for the isolation of Neisseria gonorrhoeae.

Specimens of urethral pus from 312 men with gonorrhoea were diluted and inoculated on to non-selective and selective media, the latter containing vancomycin, colistin, nystatin, and trimethoprim. Although three (1%) isolates of Neisseria gonorrhoeae were inhibited by trimethoprim 4 mg/1 and 10 by vancomycin 2 mg/1, only two failed to grow on a selective medium from large inocula but eight strai...

Methods of diagnosing gonorrhoea

Gonorrhoea is normally diagnosed presumptively by the presence of intracellular Gramnegative cocci on a Gram stain and confirmed by culture ofthe causative organism, Neisseria gonorrhoeae. Alternative methods have been evaluated extensively for the detection of gonococci in clinical specimens including immunological techniques such as ELISA and immunofluorescence, DNA probes, genetic transforma...